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| Registros recuperados: 49 | |
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| Li,Li; Mu,Lan; Wang,Xiaojuan; Yu,Jingfeng; Hu,Ruiping; Li,Zhen. |
| ABSTRACT This study aimed to describe a Bacillus subtilis expression system based on genetically modified B. subtilis. Abaecin, an antimicrobial peptide obtained from Apis mellifera, can enhance the effect of pore-forming peptides from other species on the inhibition of bacterial growth. For the exogenous expression, the abaecin gene was fused with a tobacco etch virus protease cleavage site, a promoter Pglv, and a mature beta-glucanase signal peptide. Also, a B. subtilis expression system was constructed. The recombinant abaecin gene was expressed and purified as a recombinant protein in the culture supernatant. The purified abaecin did not inhibit the growth of Escherichia coli strain K88. Cecropin A and hymenoptaecin exhibited potent bactericidal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abaecin; Antimicrobial peptides; Bacillus subtilis; Expression. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400809 |
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| Cruz-Martín,Mileidy; Sánchez-García,Cynthia; Acosta-Suárez,Mayra; Leiva-Mora,Michel; Hernández Durán,Yosbel; Roque,Berkis; Alvarado-Capó,Yelenys. |
| Se evaluó el control in vitro de Burkholderia cepacia (cepa CCIBP-E-556), Serratia plymuthica (cepa ATCC 1270) y Bacillus subtilis (cepa ATCC 6051), sobre Macrophomina phaseolina (Tassi) Goid, utilizando el método de cultivo dual.Se midió el radio de la colonia del hongo y se determinó el porcentaje de inhibición del crecimiento radial. B. cepacia produjo el mayor efecto sobre el aislado de M. phaseolina, provocando un porcentaje de inhibición del 43,5%, y ocasionando cambios en la apariencia del micelio, que se observaron en la zona de inhibición con el uso del microscópico óptico. Por su parte, S. plymuthica y B. subtilis provocaron valores de inhibición del crecimiento micelial en M. phaseolina por debajo del 10%, lo que indica que dichas cepas no... |
| Tipo: Journal article |
Palavras-chave: Actividad antifúngica; Biocontrol; Burkholderia cepacia; Serratia plymuthica; Bacillus subtilis; Frijol; Phaseolus vulgaris; Fitopatógeno. |
| Ano: 2014 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522014000100009 |
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| Machado,Alexandre Paulo; Vivi,Viviane Karolina; Tavares,José Roberto; Gueiros Filho,Frederico José; Fischman,Olga. |
| In this work, different reactions in vitro between an environmental bacterial isolate and fungal species were related. The Gram-positive bacteria had terminal and subterminal endospores, presented metabolic characteristics of mesophilic and acidophilic growth, halotolerance, positive to nitrate reduction and enzyme production, as caseinase and catalase. The analysis of partial sequences containing 400 to 700 bases of the 16S ribosomal RNA gene showed identity with the genus Bacillus. However, its identity as B. subtilis was confirmed after analyses of the rpoB, gyrA, and 16S rRNA near-full-length sequences. Strong inhibitory activity of environmental microorganisms, such as Penicillium sp, Aspergillus flavus, A. niger, and phytopathogens, such as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus subtilis; Phytopathogenic fungi; Environmental fungi; Microbial interaction. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500001 |
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| Gomes,P.A.D.P.; Bentancor,L.V.; Paccez,J.D.; Sbrogio-Almeida,M.E.; Palermo,M. S.; Ferreira,R.C.C.; Ferreira,L.C.S.. |
| No effective vaccine or immunotherapy is presently available for patients with the hemolytic uremic syndrome (HUS) induced by Shiga-like toxin (Stx) producedbyenterohaemorragic Escherichia coli (EHEC) strains, such as those belonging to the O157:H7 serotype. In this work we evaluated the performance of Bacillus subtilis strains, a harmless spore former gram-positive bacterium species, as a vaccine vehicle for the expression of Stx2B subunit (Stx2B). A recombinant B. subtilis vaccine strain expressing Stx2B under the control of a stress inducible promoter was delivered to BALB/c mice via oral, nasal or subcutaneous routes using both vegetative cells and spores. Mice immunized with vegetative cells by the oral route developed low but specific anti-Stx2B... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EHEC; Stx; Bacillus subtilis; Vaccines; HUS. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200023 |
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| Fernandes,Paulo André Vicente; Arruda,Isabel Renata de; Santos,Antônio Fernando Amatto Botelho dos; Araújo,Ana Albertina de; Maior,Ana Maria Souto; Ximenes,Eulália Azevedo. |
| Lipopeptides represent a class of microbial surfactants with increasing scientific, therapeutic and biotechnological interests. The genus Bacillus is a producer of these active compounds, and among them B. subtilis produces surfactin, the most potent biosurfactant known. These compounds can act as antibiotics, antivirals, antitumorals, immunomodulators and enzyme inhibitors. In this work, the antimicrobial activity of biosurfactants obtained by cultivation of B. subtilis R14 was investigated against multidrug-resistant bacteria. During cultivation in defined medium, the surface tension of the medium was reduced from 54 mN/m in the beginning of the microbial growth to 30 mN/m after 20 hours. A crude surfactant concentration of 2.0 g/L was obtained after 40... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biosurfactant; Bacillus subtilis; Lipopeptides; Antimicrobial activity. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400022 |
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| Méndez-Trujillo,V; Carrillo-Beltrán,M; Valdez-Salas,B; Gonzalez-Mendoza,D. |
| The aim of the present study was to isolate autochthonous microorganisms with biosurfactants capacities from the rhizospere of the cotton (Gossypium spp.), cachanilla (Pluchea sericea) and salicornia (Salicornia bigelovii) in the Mexicali valley. The biosurfactant activity and biosurfactant productions by the strains isolated from the rhizosphere of the cotton (Bs-Alg), cachanilla (Bs-Cach) and salicornia (Bs-Cach01) were determined using oil spreading technique and emulsifcation activity with corn, olive, soybean and diesel oils, respectively. The analysis of the 16S rRNA showed that strains Bs-Alg, Bs-Cach and Bs-Cach01 are closely related among them and with respect to all Bacillus subtilis with more than 99% similarity values. Results showed that all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mexicali valley; Biosurfactants; Bacillus subtilis; Native plants. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200008 |
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| Hernández-Castillo,FD; Aguirre-Aguirre,Adalberto; Lira-Saldivar,RH; Guerrero-Rodríguez,E; Gallegos-Morales,G. |
| Mediante experimentos de laboratorio e invernadero se analizó la bioeficacia de cinco cepas de Bacillus spp.; de la mezcla de los bioproductos quitosan y extracto de Larrea tridentata, así como de cinco funguicidas sintéticos contra el hongo Alternaria dauci Kühn. Además, se determinó el efecto de estos compuestos en el crecimiento del cultivo de zanahoria. Los resultados obtenidos mostraron que las cepas de la bacteria Bacillus (B3, B9, B15 y la combinación de éstas), así como la mezcla quitosan- Larrea estimularon el desarrollo de la zanahoria, ya que ésta alcanzó mayor grosor, longitud y peso, con respecto al testigo y a los demás tratamientos. La cepa B3 resultó ser la que más promovió el crecimiento de zanahoria, ya que reportó valores superiores en... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus subtilis; Biocontrol; Quitosan; Daucus carota; Larrea tridentata. |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572006000100009 |
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| Noffs,André Pedro; Tachibana,Leonardo; Santos,Antenor Aguiar; Ranzani-Paiva,Maria José Tavares. |
| The objective of this work was to evaluate the addition of Bacillus subtilis probiotic to the feed of common snook (Centropomus undecimalis) fingerlings, in alternate and continuous regimens. Six hundred and sixty fish, with average length of 5.90±0.88 cm and weight of 1.92±0.28 g, were stocked in 12 cages of 1.0 m3, with 55 fish each. The experimental design was completely randomized, with three treatments and four replicates. The treatments consisted of diet with the addition of probiotic, provided in alternate regimen for 7 days and in continuous regimen; besides a control without probiotic in the feed. Zootechnical performance, body composition, immune response, and blood parameters were evaluated. No significant differences were observed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus subtilis; Centropomus undecimalis; Hematology; Immunostimulant; Marine aquaculture; Respiratory burst.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000400267 |
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| Zhang,Wei-Wei; Yang,Ming-Ming; Li,Heng-xin; Wang,Dun. |
| As a precursor, pimelic acid plays an important role in biotin biosynthesis pathway of Bacillus subtilis. Fermentations supplemented with pimelic acid could improve the production of biotin, however, with a disadvantage-high cost. So it is necessary to improve the biosynthesis of pimelic acid via genetic engineering in B. subtilis. In this study, we constructed a recombinant B. subtilis strain for improving the synthesis of pimelic acid, in which a maltose-inducible Pglv promoter was inserted into the upstream of the cistron bioI-orf2-orf3 and, meanwhile, flanked by the tandem cistrons via a single crossover event. The copy number of the integrant was amplified by high-concentration resistance screen and increased to 4-5 copies. The production of pimelic... |
| Tipo: Journal article |
Palavras-chave: Bacillus subtilis; Biosynthesis; Integration; Pglv promoter; Pimelic acid; Single crossover event. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600001 |
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| Kupper,Katia Cristina; Bellotte,José Antonio Miranda; Goes,Antonio de. |
| A queda prematura dos frutos cítricos (QPFC), causada por Colletotrichum acutatum, dados os grandes prejuízos que têm causado aos produtores, constitui-se numa doença de grande importância econômica. O controle da doença é feito predominantemente mediante uso de fungicidas, que eleva o custo de produção e afeta negativamente o meio ambiente. Diante disso, este trabalho teve por objetivo buscar um método alternativo de controle da QPFC, mediante o uso de agentes de biocontrole ou de biofertilizantes. Diferentes concentrações de biofertilizantes (originários de duas fontes distintas e denominados de Bio1 e Bio 2); três isolados de Bacillus subtilis (ACB-69; 72 e 77) e três isolados de Trichoderma spp. (ACB-14; 37 e 39) foram testados, isoladamente ou em... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus subtilis; Trichoderma spp; Biofertilizante. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452009000400013 |
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| Cattanêo,Alex Júnior; Stangarlin,José Renato; Bassegio,Doglas; Santos,Reginado Ferreira. |
| ABSTRACT Crambe has great potential for cultivation in tropical regions, but its biotic factors may be a limiting aspect for its production. Thus, we aimed in this study to assess the effect of pre‐sowing seed treatments on seedling emergence, root system, production components, and oil. The experiment was conducted during two years in Cascavel, Paraná, Brazil. The experimental design consisted of randomized blocks with six treatments, including a control treatment (no treatment), biological treatments (Trichoderma asperellum and Bacillus subtilis), and chemical treatments (glutamic acid, thiamethoxam and zinc), with four replications. Emergence and growth crambe, as well as production components and oil in the first year were higher due to the contrast in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacillus subtilis; Trichoderma asperellum; Thiamethoxam; Glutamic acid; Zinc. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000300292 |
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| Registros recuperados: 49 | |
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